Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
- Primary Antibodies ›
- PSMB6 Antibodies
Invitrogen
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
View all (9) PSMB6 antibodies
Cite PSMB6 Polyclonal Antibody
FIGURE: 1 / 8
{{ $ctrl.currentElement.advancedVerification.fullName }}
{{ $ctrl.currentElement.advancedVerification.text }}
PSMB6 Antibody (PA1-978) in WB
Western blot was performed using Anti-PSMB6 Polyclonal Antibody (Product # PA1-978) and a 21kDa band corresponding to PSMB6 was observed across cell lines and tissue tested. Nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1), 3T3-L1 (Lane 2), PC-3 (Lane 3), MCF7 (Lane 4), A549 (Lane 5), PANC-1 (Lane 6), Mouse Kidney (Lane 7), Mouse Liver (Lane 8), Rat Liver (Lane 9) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
Published figure supplied by
{{$ctrl.videoDesc}}
{{$ctrl.videoLongDesc}}
Product Details
PA1-978
Applications
Tested Dilution
Publications
Western Blot(WB)
1 µg/mL
View 5 publications5 publications
Immunocytochemistry(ICC/IF)
1:10-1:100
-
Product Specifications
Species Reactivity
Bovine,Human,Mouse,Rat
Published species
Human,Mouse
Host/Isotype
Rabbit/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptides: C(41) R S G S A A D T Q A V/I A D A V T Y(58)
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2172197
Product Specific Information
PA1-978 detects proteasome 20S Y from purified bovine and human 26S proteasome samples.
PA1-978 has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~25 kDa protein representing proteasome 20S Y from purified human 26S proteasome.
The PA1-978 immunizing peptides correspond to amino acid residues 41-58 from human proteasome 20S Y and amino acid residues 74-91 from frog. These sequences are completely conserved in mouse, rat, and zebrafish. PA1-978 immunizing peptide (Cat. # PEP-173) is available for use in neutralization and control procedures.
Target Information
Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signaling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Other proteins are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. The PA28/11S REG Activator protein complex functions as a proteolytic activator. This complex, consisting of alpha, beta, and gamma subunits, enhances the activity of the 20S proteolytic core. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis (CF transmembrane regulator), Angelmane's syndrome (E6-AP), and Liddle syndrome (endothelial sodium channels).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases:Low molecular mass protein 19; Macropain delta chain; Multicatalytic endopeptidase complex delta chain; proteasome (prosome, macropain) subunit, beta type, 6; proteasome beta 6 subunit; proteasome catalytic subunit 1; Proteasome chain 5; Proteasome delta chain; proteasome subunit beta 6; Proteasome subunit beta type-6; proteasome subunit delta; Proteasome subunit Y; PSY large multifunctional protease Y; RP23-122P1.4; subunit 2
Entrez Gene ID:(Human) 5694, (Bovine) 510069, (Rat) 29666, (Mouse) 19175
Molecular Function: protease
Function(s)
MAPK cascadeprotein polyubiquitinationstimulatory C-type lectin receptor signaling pathwayantigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependentregulation of cellular amino acid metabolic processviral processanaphase-promoting complex-dependent catabolic processtumor necrosis factor-mediated signaling pathwayNIK/NF-kappaB signalingFc-epsilon receptor signaling pathwayproteasome-mediated ubiquitin-dependent protein catabolic processregulation of mRNA stabilityT cell receptor signaling pathwaynegative regulation of ubiquitin-protein ligase activity involved in mitotic cell cyclepositive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transitionWnt signaling pathway, planar cell polarity pathwaynegative regulation of canonical Wnt signaling pathwaypositive regulation of canonical Wnt signaling pathwaycell-cell adhesionproteolysis involved in cellular protein catabolic processproteolysisproteasomal ubiquitin-independent protein catabolic process
Process(es)
endopeptidase activitythreonine-type endopeptidase activitycadherin binding involved in cell-cell adhesionpeptidase activityhydrolase activity
It has to be done as per old AB suggested Products section.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.